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Representative immunoblots and densitometric quantification of key proteins in spleen and bone marrow: TNF-α, IL-1β, IL-6, p-IκBα/IκBα, p-NF-κB p65/NF-κB <t>p65,</t> <t>GM-CSF,</t> <t>GM-CSFr,</t> p-JAK2/JAK2, and p-STAT5/STAT5 ( A – D ). Bone marrow nucleated cell (BMNC) counts ( E ). Peripheral white blood cells (WBCs) ( F ) and lymphocyte (LYMPH) counts ( G ). Representative flow cytometry plots ( H ) and quantitative analysis of bone marrow LSK cells ( I ). Schematic of the riclin-mediated immune-hematopoietic axis ( J ). Data represent mean ± SD ( n = 3–6); * p < 0.05, ** p < 0.01, *** p < 0.001 vs CK group.
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Representative immunoblots and densitometric quantification of key proteins in spleen and bone marrow: TNF-α, IL-1β, IL-6, p-IκBα/IκBα, p-NF-κB p65/NF-κB p65, GM-CSF, GM-CSFr, p-JAK2/JAK2, and p-STAT5/STAT5 ( A – D ). Bone marrow nucleated cell (BMNC) counts ( E ). Peripheral white blood cells (WBCs) ( F ) and lymphocyte (LYMPH) counts ( G ). Representative flow cytometry plots ( H ) and quantitative analysis of bone marrow LSK cells ( I ). Schematic of the riclin-mediated immune-hematopoietic axis ( J ). Data represent mean ± SD ( n = 3–6); * p < 0.05, ** p < 0.01, *** p < 0.001 vs CK group.

Journal: NPJ Precision Oncology

Article Title: Natural polysaccharide riclin acts as an immune adjuvant to enhance chemotherapy efficacy in NSCLC

doi: 10.1038/s41698-026-01318-z

Figure Lengend Snippet: Representative immunoblots and densitometric quantification of key proteins in spleen and bone marrow: TNF-α, IL-1β, IL-6, p-IκBα/IκBα, p-NF-κB p65/NF-κB p65, GM-CSF, GM-CSFr, p-JAK2/JAK2, and p-STAT5/STAT5 ( A – D ). Bone marrow nucleated cell (BMNC) counts ( E ). Peripheral white blood cells (WBCs) ( F ) and lymphocyte (LYMPH) counts ( G ). Representative flow cytometry plots ( H ) and quantitative analysis of bone marrow LSK cells ( I ). Schematic of the riclin-mediated immune-hematopoietic axis ( J ). Data represent mean ± SD ( n = 3–6); * p < 0.05, ** p < 0.01, *** p < 0.001 vs CK group.

Article Snippet: Following antibodies were used: β-actin (3700, monoclonal), TNF-α (11948, monoclonal), IL-6 (12912s, monoclonal), and p-NF-κB p65 (3033T, monoclonal) from Cell Signaling Technology, IL-1β (sc-52012, monoclonal), granulocyte-macrophage colony-stimulating factor (GM-CSF, sc-32753, monoclonal), NF-κB p65 (sc-8008, monoclonal), phosphor (p)-IκB-α (sc-8404, monoclonal), GM-CSFr (sc-393281, monoclonal), JAK2 (sc-390539, monoclonal), STAT5 (sc-74442, monoclonal), and p-STAT5a/b (sc-81524, monoclonal) from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and p-JAK2 (AF3024, polyclonal) from Affinity Biosciences (Cincinnati, OH, USA).

Techniques: Western Blot, Flow Cytometry